Evotec provides comprehensive protein refolding services designed to restore the native structure and biological activity of recombinant proteins. Protein refolding is often required when large quantities are expressed in bacterial systems and accumulate as inactive inclusion bodies. The process involves solubilization followed by optimized refolding strategies, including dilution refolding, dialysis, and on-column chromatography. Evotec’s refolding platform enables high-yield recovery of functional proteins, ensuring scalability, reproducibility, and suitability for research and therapeutic applications.
Evotec Expertise
Evotec combines rational design with empirical screening to deliver high-yield, scalable protein refolding solutions. Proprietary methods and advanced analytics ensure reproducible recovery of biologically active proteins from inclusion bodies and denatured states.
Protein refolding workflow
- Construct design & fusion strategies for folding efficiency
- Expression optimization for recombinant proteins
- Buffer screening for denaturation/refolding conditions
- Denaturants & additives to improve solubility
- Redox control for disulfide bond formation
- Optimized pH, temperature & concentration
- Diverse refolding methods: dilution, dialysis, on-column
- Characterization with aSEC, TSA, ligand binding, DLS
Featured Protein Refolding Services
Frequently Asked Questions
Q: Why refold?
- The correct 3D protein structure is essential for biological function.
- Refolding recovers proteins from inclusion bodies or restores proteins denatured during purification.
Enables delivery of active, native proteins for research, structural studies, and therapeutic applications.
Q: What to expect from Evotec’s protein refolding services?
- Guaranteed Purity & Concentration – Typically >85% purity with validated protein concentration.
- Customization – Refolding tailored to each protein, delivered in optimized buffer conditions.
- Quality Control – SDS-PAGE, protein concentration, and additional analytical data provided.
- Technical Support – Guidance on experimental design and protocol optimization.
- Biological Activity – Refolding aims to restore native structure, with confirmation of activity recommended.
