Protein Purification

Evotec’s custom protein purification services deliver premium, fit-for-purpose proteins to accelerate innovation in structural biology, proteomics, biocatalysis, membrane protein research, and drug discovery. Powered by advanced automation and a global team of over 100 expert protein biochemists, we optimize complex purification workflows for reproducibility, scalability, and speed. Our integrated approach reduces development costs and timelines, enabling partners to confidently overcome even the most demanding protein science challenges.

Evotec offers world-class protein purification services designed to meet the demands of modern drug discovery and development. With over 100 protein biochemists with >65% holding PhDs or equivalent across three global delivery sites, our team brings over two decades of experience across diverse and complex target classes.

• Affinity chromatography, Ion exchange, hydrophobic interaction reverse phase, mixed mode and size exclusion
• Untagged and purification from native source
• Membrane proteins, integral and associated proteins
• Protein complexes including protein-protein and protein-DNA/RNA interactions
• Biocatalysis with high-throughput enzyme screening and thermo-stabilization
• Biologics production including antigens, bs-Abs, ms-Abs, Fc-fusions under endotoxin-free conditions and cGMP biomanufacturing
• High-throughput purification workflows
• Bespoke protein QC packages

Q: What types of protein purification techniques does Evotec offer?
Evotec provides a broad range of purification methods including affinity chromatography, ion exchange, hydrophobic interaction, reverse phase, mixed-mode, and size-exclusion chromatography, enabling high-quality purification across diverse protein classes.

Q: Can Evotec purify untagged proteins or proteins from native sources?
Yes. Evotec routinely purifies untagged proteins and proteins isolated directly from native sources using established purification strategies tailored to preserve activity and stability.

Q: Does Evotec handle membrane proteins and protein complexes?
Evotec specializes in purifying challenging targets such as integral and peripheral membrane proteins, including GPCRs, ion channels, and transporters, as well as protein complexes involved in protein–protein and protein–DNA/RNA interactions.

Q: Does Evotec have established high-throughput purification workflows?
Yes. Evotec uses automated ÄKTA chromatography systems with autosamplers to deliver fast, reproducible, multi-step, high-throughput protein purification suitable for complex targets and scalable research programs.

Q: What expertise does Evotec bring to protein purification?
Evotec’s purification team includes more than 100 protein biochemists, over 65 percent with PhDs or equivalent qualifications, supported by more than two decades of experience across complex and diverse target classes.

Q: Does Evotec support biologics production?
Yes. Evotec purifies biologics including antigens, bispecific and multispecific antibodies, and Fc-fusion proteins under low-endotoxin conditions, with access to cGMP antibody manufacturing when required.

Q: Are custom quality control (QC) packages available?
Evotec offers bespoke QC packages tailored to project needs, ensuring each purified protein meets required specifications for structural, biophysical, or functional studies.

Q: Where are Evotec’s protein purification services delivered from?
Evotec delivers non-GMP protein purification from sites in Abingdon (UK), Princeton (US), and Hamburg (Germany), with cGMP biologics manufacturing supported from Seattle and Redmond in the US to ensure global scalability and flexibility.