Grating coupled interferometry is a cutting-edge biophysical characterization approach which offers superior data quality for studying biomolecular interactions. The technique is label-free and it quickly and accurately measures binding affinity and kinetics.
Grating Coupled Interferometry (GCI) Technology
Grating-coupled interferometry (GCI) is a biosensor technology able to achieve superior resolution in signal and time compared to traditional Surface Plasmon Resonance (SPR). The microfluidic cartridge is embedded into the disposable sensor chip, with a no-clog technology that allows injection of crude samples, harsh chemicals, and particles up to 1000 nm. These features, combined with higher flexibility in the experimental design – exemplified in the RAPID (Repeated Analyte Pulses of Increasing Duration) injection scheme – render GCI attractive for the study of transmembrane proteins.
At Evotec, GCI technology is routinely used for direct target engagement studies, binding kinetics quantification, and biophysical fragment screening on different classes of transmembrane proteins as GPCRs, SLCs, ion channels, tetraspanins and integral membrane enzymes.
An example of the R&D work on proprietary GPCRs conducted by Evotec can be exemplified in the joint webinar with Malvern Panalytical:
Fragment screening of GPCRs using Grating Coupled Interferometry (GCI) technology
Learn More in Our Webinar
