Surface Plasmon Resonance (SPR) Services

Surface Plasmon Resonance (SPR) evaluates and characterizes drug-target interactions and forms part of Evotec's biophysics biosensor-based services. The surface plasmon resonance technology can be applied to various modalities including small molecules, peptides, biologics and oligonucleotides.

Surface plasmon resonance technology is regularly applied in all kind of drug discovery approaches to characterize drug-target interactions including biophysical fragment screening, validation of lead like small molecule hit compounds, peptides, biologics and oligonucleotides. While many targets are within the protein space, Evotec also tests binders against RNA and antibodies. Evotec can offer a variety of immobilization technologies depending on drug-target interaction and kinetic resolution. The SPR assay technology allows for the kinetic characterization of drug-target interaction but can also provide structural information with an epitope binning approach.

Recent case studies applying SPR technology have been published with collaborating partners:

Forced self-modification assays as a strategy to monoPARP enzymes

Discovery of potent SOS1 inhibitors that block RAS activation via disruption of the RAS–SOS1 interaction

Evotec offers ready-to-use biologics SPR assays with reduced assay development time and delivery of high data quality. Optimized and standardized workflows are available on Evotec's Biacore 8K+ platforms to support antibody characterization by SPR from early-stage discovery to lead optimization.

• Off-rate ranking of antibodies from phage display crude or hybridoma soup
• Direct binding against antigen with full kinetic profile
• Cross-reactivity direct binding assays
• Epitope binning (Sandwich, Pre-mix, and/or Tandem)
• FcRn pH dependency (available on multiple species) as off target assay
• FcγR receptors (available on multiple species) as off target assay
• Simultaneous binding (e.g. bispecific antibodies)
• Competition assays
• Evotec's automated workflows include the application of robot liquid handling devices to increase robustness of assay procedure.