In orthogonal screening, a different technology or assay is used to re-confirm the hits. This could be a biophysical assay to confirm direct binding of hits to the target and assess their mode-of-action.
Biophysics Orthogonal Technologies
Various other biophysical techniques like DSF, MST or nephelometry are complementing the biophysical platform to select the best approach for Evotec's drug discovery programs.
- Differential Scanning Fluorimetry (DSF) applications:
- Protein QC and buffer screening
- Screening of lead like collection with up to 50k compounds
- Hit triaging for x-ray crystallography
- Microscale Thermophoresis (MST):
- Direct binding assay in solution with labeled and label-free approach
- Competition assays
- Nephelometry:
- Testing of compound solubility
- Aggregation assays
Additionally, Evotec can access further biophysical low throughput techniques like mass photometry, ITC, or CD in collaboration with EMBL SPC, Hamburg
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