Market leading perfusion medium for high biotherapeutic productivity and robust scalability in CHO cell culture platforms.
J.Media™ is a Chemically-defined (CD), animal origin free (AOF), HEPES free cell culture media designed to support high productivity and robust product quality from CHO cell lines from development to manufacturing scale. Optimized for perfusion-based upstream processes, J.Media™ is room temperature stable, concentrate-ready and available in liquid and powder formats for large-scale biomanufacturing applications.
J.Media Compared to Other Commercial Media
Key Features of Just - Evotec Biologics' CHO Perfusion Medium
- J.Media features superior storage properties
- J.Media is available as a 5x concentrate powder, reducing storage requirements, essential for scaled‑up perfusion processes that require large volumes of media.
- In its liquid format, J.Media is stable for up to 30 days at 1× concentration and up to four months at 5× concentration.
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Benchmarking in Small Scale Mock Perfusion Case Study
As part of a small scale mock perfusion study performance was compared to commercially available perfusion media using two IgG1-expressing CHO cell lines (J.CHO®). Performance scalability was tested from 3 L bench scale to 500 L single use bioreactors (SUB). Results show market-leading volumetric and specific productivity by efficiently funneling nutrients towards protein production while maintaining consistent critical quality attributes (CQAs) including glycans such as high mannose, sialylation, β-galactose, and afucosylation.
J.Media™ drives higher mAb1 and mAb2 volumetric productivity in comparison to commercial perfusion media and supports highest cumulative titer over days for both mAb1 and mAb2.
Figure 1. Mock Perfusion cell culture parameters.
CHO-GS cells expressing mAb1 and mAb2 were grown in 24 DWP at working volume 2 mL. (A–D) Blue line, circle: J.Media™ medium, black line, triangle: Medium A, black line, square: Medium B, black line, diamond: Medium C. Shaded region shows the confidence interval (α = 0.05). A) Specific productivity (qp), (B) Volumetric productivity (Vp), (C) Viable cell density (VCD), (D) Viability (%), and (E) Cumulative titer data is shown for 3 technical replicates each for J.Media™ medium and three commercial perfusion media, where letters indicate statistically different groups based on Tukey’s HSD test. Blue bar: J.Media™ medium; white bars: commercial media A. B, and C.
In the study we demonstrate that J.Media™ medium can support the desired cell growth, productivity, and quality of CHO cells to produce best-in-class protein production for two IgG1 molecules tested.
We also demonstrated scalability to clinical/commercial levels using J.Media™ medium. Notably, 500 L scale bioreactor runs in this study utilized a 5× concentrate with feeds shelf stable at room temperature. These two attributes – the ability of the proprietary J.Media™ medium to concentrate to 5× and its long-term stability at room temperature – are important for commercial manufacturing considerations such as cold chain storage, transport and shelf life. Both perfusion formats and large scale fed batch (>5000 L) have large media requirements and as such J.Media™ medium use is also highly desirable from a facility footprint utilization viewpoint.
In summary, Just - Evotec Biologics' CHO Perfusion Medium (J.Media) demonstrates superior performance in case study:
- Highest specific productivity in comparison to commercial media in mAbs tested in small scale mock perfusion
- High density (>80 million cells/mL), high viability perfusion processes over at least 15 days
- Robust scaled up performance: Low impurity and consistent glycosylation profiles from bench top to large scale
