Evotec’s protein labeling and conjugation platform delivers high-quality, customized proteins for structural biology, proteomics, and drug discovery. Capabilities include uniform and amino acid–selective stable isotope labeling, non-natural amino acid incorporation, and site-specific conjugation such as biotinylation to enhance analytical performance and functional versatility. Using bacterial, yeast, insect, and mammalian systems, Evotec produces isotopically and chemically modified proteins at scalable yields with high purity and functional validation. The team ensures optimized construct design, expression, and purification workflows to support NMR, mass spectrometry, and mechanistic studies with reliable, reproducible results.
Evotec Expertise
Evotec combines rational design with empirical screening to deliver high-yield, scalable, stable isotope-labelled and non-natural amino acid proteins. Proprietary methods and advanced analytics ensure reproducible high-level incorporation and recovery of active proteins.
- Stable isotope labeling including uniform 13C, 15N, 2H and amino-acid selective labeling
- Non-natural amino acid incorporation for expanded functionality
- Site-specific conjugation such as biotinylation and fluorescent tagging
- Flexible production across cell-free, E. coli, yeast, insect, and mammalian systems
- Scalable yields from milligram to gram quantities
- High purity with full functional validation
- Applications in NMR screening, structural analysis, quantitative proteomics, biophysics, and assay development
Protein labeling and conjugation workflow
Featured Protein Labelling & Conjugation Services
Frequently Asked Questions
Q: What types of protein labelling does Evotec offer?
Evotec provides uniform 13C, 15N, and 2H stable isotope labelling, amino acid-selective labelling, non-natural amino acid incorporation, and site-specific conjugation such as biotinylation and fluorescent tagging.
Q: Which expression systems are available for protein production?
Proteins can be produced using cell-free systems, E. coli and other bacteria, yeast such as Pichia pastoris, insect cells including Sf9 and Sf21, and mammalian hosts like HEK293 and CHO.
Q: What are the typical applications of labelled proteins?
Labelled proteins support NMR and structural biology, quantitative proteomics and mass spectrometry, biophysical studies including SPR and ITC, and assay development or mechanistic investigations.
Q: Can Evotec scale production for larger quantity needs?
Yes. Evotec offers scalable production from milligram to gram quantities with consistent, reproducible performance across batches.
Q: How does Evotec ensure protein quality and functionality?
All proteins undergo high-purity purification, functional validation, and advanced analytical characterization to confirm labelling efficiency and activity.
Q: Do you offer custom construct design and cloning?
Yes. Evotec provides tailored construct design, cloning, and expression optimization based on the target protein and application requirements.
Q: What makes Evotec’s protein labelling and conjugation services unique?
Evotec delivers proprietary high-incorporation methods, empirical expression screening, multi-system expertise, and a proven track record of reliable, reproducible results.
