Evotec’s Protein Characterization Platform delivers comprehensive analytical solutions to assess protein purity, identity, homogeneity, stability, post-translational modifications, activity, and antibody-specific attributes. Leveraging state-of-the-art Protein Characterization Equipment and deep scientific expertise, the platform provides high-quality, reproducible data to support structural biology, biologics development, and biomanufacturing. Integrated workflows ensure reliable characterization across a wide range of recombinant, engineered, and therapeutic proteins.
Evotec Expertise
Evotec delivers comprehensive protein characterization using advanced analytics and empirical screening to evaluate purity, identity, homogeneity, stability, post-translational modifications, activity, and antibody attributes, ensuring high-quality, reproducible data for structural biology, biologics development, and formulation.
- SDS-PAGE, capillary electrophoresis, A280, BCA/Bradford, and HPLC-aSEC provide a precise assessment of purity, concentration, monomer content, and aggregation
- Western blotting, intact mass spectrometry, and peptide mass fingerprinting confirm molecular identity and sequence integrity
- aSEC/fSEC, DLS, SEC-MALLS, and negative-stain EM evaluate sample homogeneity, aggregation state, and particle morphology
- DSF and nanoDSF measure thermal stability and melting transitions under native or dye-free conditions
- Gel-shift assays and covalent labeling mass spectrometry map and quantify post-translational modifications
- Plate-based activity assays, SPR, GCI, and ITC determine functional activity, binding kinetics, affinity, and thermodynamics
- Intact mass analysis, charge variant profiling, UPLC-SEC, glycan analysis, HIC, and Protein A quantification support detailed antibody characterization
Protein characterization workflow
Featured Protein Characterization Services
Frequently Asked Questions
Q: What does Evotec’s protein characterization platform offer?
Evotec provides a comprehensive suite of analytical methods to assess protein purity, identity, homogeneity, stability, post-translational modifications, activity, and antibody-specific attributes.
Q: How is protein purity and concentration measured?
Purity and quantity are evaluated using SDS-PAGE, A280 UV absorbance, BCA or Bradford assays, and HPLC-SEC for accurate monomer and aggregate analysis.
Q: How do you confirm protein identity?
Identity is verified through Western blotting, intact mass spectrometry, and peptide mass fingerprinting.
Q: What methods assess protein homogeneity?
Analytical SEC, DLS, SEC-MALLS, and negative-stain electron microscopy are used to evaluate sample uniformity and detect aggregation.
Q: How is protein stability evaluated?
Thermal stability and unfolding behavior are measured with dye-based DSF and label-free nanoDSF.
Q: Do you analyze post-translational modifications?
Yes. PTMs are assessed using gel-shift assays and mass spectrometry to identify and quantify covalent modifications.
Q: What functional assays are available?
Evotec offers plate-based activity assays and binding analysis using SPR, GCI, and ITC for kinetic and thermodynamic characterization.
Q: Do you specialize in antibody characterization?
Yes. Capabilities include intact mass analysis, charge variant profiling, glycan analysis, UPLC-SEC, hydrophobic interaction chromatography, and antibody titer quantification.
