Introduction

Epilepsy is a neurological disorder characterized by recurrent and sustained neuronal discharges. Different approaches have been used to evoke seizure-like activity but chemoconvulsants are the most used. In this work, we challenged mouse hippo-cortical slices with different compounds. Activity evoked by acute application of these compounds was monitored through high-density multi electrode array (HD-MEA) in order to characterize in time and space seizure like activity. In particular we challenged slices with kainic acid (KA, 10 μM) and the high-selective agonist (RS)-2-Amino-3-(3-hydroxy-5-tert-butylisoxazol-4-yl) propanoic acid (ATPA, 10 μM) in order to acivate kainate receptors. Furthermore, we compare the effect of these compounds with the convulsant 4-aminopyridine (4-AP, 100 μM), the voltage-dependent potassium channels blocker.

_{1 Aptuit Srl, an Evotec Company, Verona, Italy}

_{2 3Brain AG, Wadenswil, Switzerland}