Introduction

Parkinson disease (PD) is a progressive neurological disease caused by selective loss of dopaminergic neurons in the substantia nigra. Although the majority of PD cases are sporadic, familial PD mutations provide a valuable tool for understanding and modelling basic pathophysiological mechanisms. We used MyCell® DopaNeurons carrying the A53T mutation in the SNCA gene (A53T DaNs) and healthy isogenic control iCell® DopaNeurons (WT DaNs) to investigate disease-relevant phenotypes including:

• Alpha synuclein (αSyn) accumulation
• Mitochondrial dysfunction
• Calcium dysregulation