Join us in-person for the PepTalk (Re)Discovering Protein Expression Platforms. Learn more about our involvement in the event below.
Just - Evotec Biologics at PepTalk (Re)Discovering Protein Expression Platforms
Bispecific Antibody Production Using Split Selectable Markers through mRNA Trans-Splicing
Date and Time: Monday, 19 January at 3:30 pm
Speaker: Yiting Lim, PhD, Senior Scientist II, Cell Line Development, Just - Evotec Biologics
To overcome challenges in multi-chain and bispecific antibody expression, we created a new stable expression system using only the auxotrophic glutamine synthetase (GS) selection marker. Up to four different antibody chains can be stably expressed in GS-KO CHO-K1 cells using two plasmids in our split GS vector system through mRNA trans-splicing, a post-transcriptional event whereby exons from two separate RNAs join to produce a chimeric RNA.
