About the Webinar
High performance cell culture media provide an essential environment to ensure cells are producing high titers and high quality biotherapeutics. These attributes are important drivers in determining whether a biotherapeutic can be manufactured commercially with economics that support market launch.
In this webinar, Just-Evotec Biologics describes their cell culture media, called J.Media, which is a chemically-defined (CD), animal origin free (AOF) Chinese Hamster Ovary (CHO) perfusion cell culture medium designed to maximize productivity and cell viability across scales, while maintaining critical product quality attributes. The proprietary formulation is room temperature stable and able to be concentrated 5x making it attractive for clinical and commercial use at both small and large scale.
J.Media performance was compared to commercially available perfusion media using two IgG1-expressing CHO cell lines in small scale mock perfusion study, and performance scalability was tested from 3 L bench scale to 500 L single use bioreactors (SUB). Results show market-leading volumetric and specific productivity by efficiently funneling nutrients towards protein production while maintaining consistent critical quality attributes (CQAs) including glycans such as high mannose, sialylation, β-galactose, and afucosylation.
Key Takeaways:
- CHO cell culture medium optimized for perfusion.
- Available as liquid for pilot scale and powder for large scale manufacturing.
- Powder is stable up to 24 months at room temperature.
