Join us in-person in San Diego Convention Center at 74th ASMS Conference. Learn more about our involvement in the event below.
Evotec at 74th ASMS Conference
Poster Presentations
A Sweet Solution for Mass Spectrometric Glycan Analysis
Glycans can significantly influence the stability, activity, and immunogenicity of biologics, making their analysis crucial for assessing the quality of antibody therapeutics, especially biosimilars. Presented here is a tool to streamline mass spectrometry-based glycan analysis automated glycan grouping with software. From released glycans to peptide-level multi-attribute method (MAM) workflows, automated glycan grouping and intuitive visualizations can transform complex datasets into actionable insights. Work presented here shows the utility of glycan grouping in bridging data from multiple analytical methods such as MAM and released glycan analysis. Additionally, glycan grouping was used to compare day of culture samples from different bioreactors using mass spectrometry (MS)
Presented by Kiran Iyer, Senior Scientist I, Process and Product Design, Mass Spectrometry, Just - Evotec Biologics
Sensitive and Robust Quantification of High-Risk Host Cell Protein by Mass Spectrometry Using Pierce™ Critical HCP Heavy AQUA Peptides
Chinese hamster ovary cells (CHO cells) are engineered to produce biotherapeutics such as monoclonal antibodies (mAb). CHO cells also express endogenous host cell proteins (HCPs). These process-related impurities, even at low ppm levels, can potentially impact product quality and safety due to immunogenicity, biological or enzymatic activities. The analytical challenges and current methodology to identify these residual host cell proteins are documented in USP <1132.1>. We utilized Pierce™ Critical HCP Heavy AQUA Peptide stable isotope labeled synthetic peptides in our HCP workflows using native digestion sample preparation. HPC65 allows confirmation of sub-ppm HCP and improves quantitative precision and repeatability. Using targeted mass spectrometry, we can achieve sensitivity needed to confirm clearance of selected high-risk HCP in final drug substance.
Presented by Eric Huang, Senior Scientist I, Process and Product Design, Mass Spectrometry, Just - Evotec Biologics
High Throughput Cysteine Chemoproteomics Workflow Enables Rapid Screening of Electrophilic Fragments at Proteome Scale
Presented by Catherine Pech, Group Leader, Translational Proteomics and Metabolomics
Date: Monday, June 1
Topic area for poster: High Throughput MS
Multi omics Profiling of Autoimmune Conditions Reveals Novel Potential Biomarker Candidates
Presented by Michaël Méret, Senior Research Scientist, Metabolomics
Date and Time: Wednesday June 3, 2:30 pm - 4:30 pm
Location: WOD, Ballroom, Metabolomics Untargeted Approaches Session
