Non-CYP Mediated Metabolism

Monoamine Oxidase (MAO) Reaction Phenotyping Assay

Understand if your compound is metabolized by MAO-A or MAO-B enzyme.

Monoamine oxidase (MAO) reaction phenotyping is a non-CYP mediated metabolism assay within our portfolio of in vitro ADME screening services. Cyprotex delivers consistent, high quality data with the flexibility to adapt protocols based on specific customer requirements.


Determining potential metabolism by MAO enzymes:

  • Monoamine oxidases (MAO) are membrane-associated enzymes located specifically on the outer mitochondrial membrane. They are the major enzymes participating in the catabolism of monoamine neurotransmitters and related exogenous amines.
  • Two isoforms of MAO exist, MAO-A and MAO-B, which differ in their substrate specificity, inhibitor selectivity and tissue distribution.
  • Selective MAO-A inhibitors are useful in the therapy of depression and anxiety whereas MAO-B inhibitors are often used in the treatment of Parkinson’s and Alzheimer’s diseases.
  • Cyprotex’s MAO reaction phenotyping assay identifies if your compound is a substrate for either MAO-A or MAO-B.


Monoamine Oxidase (MAO) Reaction Phenotyping Assay Protocol


Data from the Monoamine Oxidase (MAO) Reaction Phenotyping Assay

Mao reaction phenotyping fig 1

Figure 1
Metabolic stability of the MAO substrate, kynuramine over 45 min in the presence of MAO-A and MAO-B expressed enzyme (Each data-point is mean of n=3).


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Cyprotex enables and enhances the prediction of human exposure, clinical efficacy and toxicological outcome of a drug or chemical. By combining quality data from robust in vitro methods with contemporary in silico technology, we add value, context and relevance to the ADME-Tox data supplied to our partners in the pharmaceutical or chemical industries.