Nuclear magnetic resonance (NMR) spectroscopy is an important technique for studying biomolecular interactions and it forms part of Evotec's biophysical assay services.
NMR Spectrometry for Binding Interactions
Bio-NMR spectroscopy is among the most sensitive techniques to confirm binding interactions between a ligand and a macromolecule. Evotec is applying NMR-approaches not only during initial hit identification activities like biophysical fragment screening and hit validation, but also in support of drug discovery activities for new modalities like RNA- or antibody-based research. Evotec's NMR platform is equipped with a Bruker 600MHz Avance III HD spectrometer, including 1.7mm TCI and 5.0 mm TXI Cryo-probeheads.
Ligand- and protein-observed NMR techniques are applied for biophysical fragment screening and binding validation activities. Selection of the appropriate NMR method depends on several factors like molecular weight of the target or protein quantities available. The bio-NMR team will provide recommendations regarding the selection of most suitable methods.
The bio-NMR team is supported by a specialized protein production team with a focus on production of isotope labeled proteins for bio-NMR experiments. The protein production team is working together closely with the global reagent production unit, applying similar streamlined work-flows.
Furthermore, Evotec can offer 19F-NMR capabilities and can access to additional NMR infrastructure (e.g. NMR instruments at very high field) in a long-standing collaboration with the Leibniz-FMP Berlin: Peter Schmieder | Leibniz-FMP
Published NMR Case Studies
See our recent published case studies applying the bio-NMR technology with various collaborating partners:
Ligandability assessment of the C‐terminal Rel‐homology domain of NFAT1
Fragment-based discovery of DNA gyrase inhibitors targeting the ATPase subunit of GyrB
Learn More in Our Webinar
