3D Microtissue & Multilayer Models

ATP Content in 3D Microtissues


  • The environment created by a 3D culture model allows reconstitution of the natural cellular physiology by promoting the complex cell-cell and cell-matrix network communications found in vivo.
  • Different scaffold-free 3D microtissue models are available at Cyprotex including spheroids produced using ultra-low adhesion plates.
  • Microtissues can be formed from single or co-cultured cell populations and their longevity permits use in long-term repeat dose toxicity studies to investigate any cumulative effect.
  • ATP content is a biochemical endpoint used to measure general cell viability. This can be incorporated with LDH release to monitor cell death over time.


ATP Content in 3D Microtissues


Data from ATP Content in 3D Microtissues Assay

ATP content microtissues 1

Figure 1
Determination of cellular ATP levels using Promega’s CellTiter Glo® luminescent cell viability assay, following exposure of 3D microtissues to rosiglitazone.


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Sam Bevan

Sam Bevan

Principal Scientist

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Cyprotex enables and enhances the prediction of human exposure, clinical efficacy and toxicological outcome of a drug or chemical. By combining quality data from robust in vitro methods with contemporary in silico technology, we add value, context and relevance to the ADME-Tox data supplied to our partners in the pharmaceutical or chemical industries.