Mass spectrometry (MS)-based ubiquitinomics allows a system-level understanding of ubiquitin signaling.

In this publication, we focus on:

• A background to ubiquitinome profiling
• Presentation of a scalable and robust workflow for deep and precise in vivo ubiquitinome profiling using DIA-MS (data-independent acquisition mass spectrometry) and neural network based data processing
• Comprehensive mapping of substrates of deubiquitinase USP7, an anticancer drug target known to regulate tumor suppressor p53
• Application of the method including rapid mode of action profiling of candidate drugs targeting deubiquitinases or ubiquitin ligases