Apoptosis & Necrosis Assay (Flow Cytometry)
Detect compound induced cell death using Cyprotex’s flow cytometric apoptosis and necrosis assay.
Cyprotex delivers consistent, high quality data with the flexibility to adapt protocols based on specific customer requirements.
Introduction
Background information:
- Flow cytometry allows analysis of multiple parameters of cell health and provides a high throughput analysis of compound cytotoxicity.
- Apoptosis and necrosis occur during cell death in response to cytotoxic conditions. Cyprotex’s apoptosis and necrosis assay utilizes Annexin V and propidium iodide dual staining to monitor cellular death.
- Annexin V-FITC binds to phosphatidylserine which is translocated from the inner to outer plasma membrane during early apoptosis. Propidium Iodide is a cell impermeable nuclear dye which is excluded by viable and early apoptotic cells. However, it is taken up by necrotic or late apoptotic cells resulting in red fluorescence.
- A dose dependent increase in apoptotic or necrotic cells, accompanied by a decrease in live cells, indicates a compound is inducing cellular death.
Protocol
Protocol for Apoptosis & Necrosis Assay
Data
Data from Cyprotex's Apoptosis & Necrosis Assay
References
1) Elmore SA et al., (2016) Recommendations from the INHAND Apoptosis/Necrosis Working Group. Toxicologic Pathology 44(2); 173-188
Contact Our Experts
Cyprotex enables and enhances the prediction of human exposure, clinical efficacy and toxicological outcome of a drug or chemical. By combining quality data from robust in vitro methods with contemporary in silico technology, we add value, context and relevance to the ADME-Tox data supplied to our partners in the pharmaceutical or chemical industries.
