UPLC Mass Spectrometry

Evotec offers UPLC Mass Spectrometry services as part of its extensive biophysics offering. The technique is used for QC purposes to assess quality of screening hits (using UPLC-MS), and for quantitative analysis and separation purposes (using UPLC-MS/MS).

Evotec's UPLC/MS-Quality control (“QC”) ensures both, high-quality compounds along the life cycle of a library (entry- & rolling QC) as well as the quality of screening hits (spot checks). Waters Acquity UPLC instruments are coupled to TQD and QDA systems with ESI(+/-) ionization, parallel UV and optionally ELS detection to analyze purity and identity of internal and external small molecules. Evotec's data evaluation follows a reproducible automated processing logic with manual review by exception. Results are reported in an electronic format that is customizable to client database input needs. A range of sample sizes can be handled up to 100k compounds per year.

Triple-quad systems are also used for quantitative MS/MS-analysis that need UPLC separation, e.g. to separate complex matrices from the target analyte.