Toxicology

Cyprotex ADME PK Toxicology In Silico Physicochemical Profiling

Toxicology

Cyprotex can assist you in understanding the toxic liability of your compounds using a panel of different techniques. We can help identify which compounds have the optimal ADME and safety profile to advance into the clinic. Our focus on state of the art technology and automation allow for high quality data to be generated rapidly and cost-effectively.

We run several high-content-screening (HCS) platforms that use automated fluorescence imaging to simultaneously analyse multi-parametric indicators of cytotoxicity. This improves the prediction of toxicological events and allows for a better understanding of the mechanisms of drug toxicity.

Our listed services can be found below. Can't see the assay you need? Please contact us with your requirements.

Multiparametric Toxicity

  • Cytotoxicity screening panel
  • CellCiphr® Premier

Cardiotoxicity

  • hERG safety
  • eCiphr®Cardio — iPS cell-derived cardiomyocytes and MEA
  • Structural cardiotoxicity assay — using 3D spontaneously beating microtissues
  • Combined hypertrophy and cardiotoxicity assay — using 3D spontaneously beating microtissues
  • Cardiotox screen — cardiac safety liability assay

Neurotoxicity

  • eCiphr®Neuro
  • eCiphr®Neuro-Human
  • Neurite outgrowth
  • 3D brain microtissue combined with high-content-imaging (HCI) end points

Nephrotoxicity

  • Chronic exposure nephrotoxicity assay

Hepatotoxicity & Drug-induced Liver Injury (DILI)

  • Mitochondrial dysfunction
  • Reactive oxygen species 
  • Oxidative stress 
  • Cytochrome P450 time dependent inhibition
  • Reactive metabolite assessment
  • Transporter interactions
  • Lysosomal trapping
  • Phospholipidosis and steatosis
  • Genotoxicity
  • Transcriptomics analysis
  • Cytotoxicity screening panel
  • CellCiphr Premier
  • 3D hepatotoxicity assay using HepaRG spheroids
  • Cholestasis

Immunotoxicology

  • PBMC cytotoxicity assay
  • PBMC proliferation assay

3D Microtissue and Spheroid Models

  • High-content-imaging (HCI) of 3D microtissues
  • ATP content in 3D microtissues
  • Structural cardiotoxicity assay using 3D spontaneously beating microtissues
  • Combined hypertrophy and cardiotoxicity assay using 3D spontaneously beating microtissues
  • 3D hepatotoxicity assay using HepaRG spheroids
  • 3D brain microtissue combined with high-content-imaging end points

Mitochondrial Toxicity

  • Functional mitochondrial toxicity assay (Seahorse)
  • Mitochondrial respiratory complex assay (Seahorse)
  • Mitochondrial biogenesis assay
  • Mitochondrial Glu/Gal assay
  • HCS mitochondrial toxicity
  • Mitochondrial oxidative stress

Custom Toxicology Service

  • Custom transcriptomics services

Mechanistic Toxicity

  • Cell stress panel
  • Apoptosis and necrosis assay
  • Phospholipidosis
  • Lysosomal trapping
  • Haemolysis
  • Reactive metabolite assessment
  • Cell viability (MTT, LDH and neutral red)
  • Toxicological gene regulation
  • Custom toxicology
  • Phototoxicity
  • KeratinoSens™ for skin sensitisation
  • Androgen receptor modulation assay

Non-GLP Exploratory Toxicology

  • Early assessment of drug safety risk
  • Mechanistic toxicology studies
  • Proof of concept for drug mechanism of action or efficacy

Drug-drug Interactions

Genotoxicity
  • Ames test
  • In vitro micronucleus test (HCS CHO-K1 cells)
  • In vitro micronucleus test (Flow cytometry TK6 cells)
  • pH3 and pH2AX genotoxicity assay
  • γH2AX double strand DNA damage response assay
  • Custom toxicology

Can't see the assay you need? Please contact us with your requirements.

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