Our high throughput screening of cellular and biochemical assays is conducted in 1,536-well and 2,080-well plate formats on three proprietary and fully integrated screening systems (EVOscreen™).
The open architecture of these high throughput screening platforms allows the incorporation of:
- Proprietary Insight FCS+plus readers
- Opera™ confocal cell imaging device
- Commercial multimode readers (e.g. EnVision™ readers)
- Various liquid handling devices
FCS+plus is a portfolio of confocal fluorescence readouts including:
- Fluorescence intensity
- Anisotropy
- Lifetime and powerful combinations thereof with a single molecule resolution
A BasePlate™ system (The Automation Partnership) is the core element of compound reformatting, cherry picking and compound dilution. The proprietary data analysis software 'A+' tracks the entire screening process from raw screening data to final data analysis and summary reports.
Evotec offers high throughput screening of its own compound collection and / or client collections. A typical high throughput screen project encompasses the adaptation of the assay to a high throughput screening format, pilot testing of a sub-set of compounds, primary screening in singlicates with on-plate performance controls, statistical hit-identification, hit-confirmation, IC50 profiling, secondary testing, structural verification of the hit-compounds and SAR analyses analyses. All data is captured in a comprehensive written report submitted as part of the service offered.