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Electrophysiology Platform

Patch-clamp electrophysiological investigations of ion channel function:

for HTS follow-up
during ion channel lead identification and optimization projects

Automated patch-clamp robot and manual rigs
Investigations on voltage and ligand-gated channels
Stably or transiently transfected and primary cells (e.g. hippocampal neurons) available
Safety pharmacology investigations on all major cardiac ion channels, including hERG (GLP optional)
Specificity, potency & selectivity profiling
Determination of mechanism-of-action
Tailored protocols for specific customer needs, or standard protocols for routine testing available

Patch-clamp electrophysiology is the 'gold standard' to investigate ion channel function.

Evotec has a proven track record of success in ion channel screening services. This includes electrophysiological investigations performed for more than 80 clients worldwide, including major Pharmaceutical and Biotechnology companies.

Studies are performed on compounds provided by our clients against a wide range of voltage and ligand-gated ion channels. Evotec's electrophysiology platform consists of a number of manual rigs, a semi-automated planar patch device (Port-a-Patch^© NPC^©-1) and an automated patch-clamp robot (PatchXpress^® 7000A) to offer high throughput screening (i.e. simultaneous testing of up to 16 compounds) and has the advantage of facilitating high quality compound characterization much earlier in the drug discovery process than was previously possible.

In addition to our electrophysiology platform, Evotec also offers a wide range of complimentary capabilities including:

ADMET & in vitro safety pharmacology
Library of ion channel cell lines and cDNAs available
Ion channel focused compound collection available for screening
HTS using fluorescence-based functional assay system
Assay development and automated high throughput screening
Medicinal chemistry expertise from hit to preclinical candidate

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